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当前位置: 首页 > 产品中心 > Magnetic_beads > 牛津生物医学研究/9(+)-HODE EIA试剂盒/试剂盒/EA80
商品详细牛津生物医学研究/9(+)-HODE EIA试剂盒/试剂盒/EA80
牛津生物医学研究/9(+)-HODE EIA试剂盒/试剂盒/EA80
牛津生物医学研究/9(+)-HODE EIA试剂盒/试剂盒/EA80
商品编号: EA80
品牌: oxfordbiomed
市场价: ¥6800.00
美元价: 3400.00
产地: 美国(厂家直采)
公司:
产品分类: 磁珠
公司分类: Magnetic_beads
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍
Product Overview

Background

Linoleic acid is the predominant polyunsaturated fatty acid (PUFA) in the human diet. The hydroxyoctadecadienoic acid (HODE) derivatives of linoleic acid, 9(S)-HODE, 9(R) HODE, and 13(S)-HODE, are the most widely distributed of the known linoleic acid metabolites. HODEs can be generated enzymatically by cyclooxygenase, lipoxygenase, and some cytochrome P450s. However, the bulk of linoleic acid peroxidation, especially generation of 9-HODEs, occurs by non-enzymatic processes so that HODEs are excellent indicators of free radical mediated lipid peroxidation. In mammalian cells, free radicals are derived primarily from peroxide and singlet oxygen, which are referred to as reactive oxygen species (ROS). ROS-derived free radicals include the super-oxide anion and the hydroxyl radical. Most ROS diffuse only a nanometer or so before reacting. Among the most important targets of free radical reactions in vivo are lipids, and resultant oxidized lipids can travel throughout the circulatory system.

 

 

Assay Principle

This kit is a competitive enzyme-linked immunoassay (ELISA). Briefly, the 9-HODE present in the samples or standards competes with 9(±)-HODE conjugated to horseradish peroxidase [9(±)-HODE-HRP] for binding to anantibody specific for 9(±)-HODE that is precoated on a microplate. The peroxidase activity of 9(±)-HODE-HRP results in color development when a substrate is added. The intensity of the color is proportional to the amount of 9(±)-HODE-HRP bound and is inversely proportional to the amount of unconjugated 9-HODE present in the samples or standards.

 

 

 

 

 

 

Spec Sheet
EA80.090304.pdf
品牌介绍
Oxford Biomedical Research(OBR)公司由Denis M. Callewaert博士于1984年建立。OBR公司开发,生产和销售检测试剂盒,重组蛋白和应用于生物医学和制药研究的抗体,现已具有400多种产品。我们产品的重点是对引发多种疾病的两大风险因素氧化应激和慢性炎症的生物标志物的识别与检测。我们采用专有技术,开发组织和疾病的特异性生物标志物。