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商品详细牛津生物医学研究/雄烯二酮EIA试剂盒/试剂盒/EA68
牛津生物医学研究/雄烯二酮EIA试剂盒/试剂盒/EA68
牛津生物医学研究/雄烯二酮EIA试剂盒/试剂盒/EA68
商品编号: EA68
品牌: oxfordbiomed
市场价: ¥4900.00
美元价: 2450.00
产地: 美国(厂家直采)
公司:
产品分类: 文库
公司分类: Gene_library
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍
Product Overview

Background

Androstenedione is synthesized in the adrenal gland. It is the primary precursor of testosterone in women. Therefore, in women, androstenedione contributes significantly to the level of androgenic activity, while in men this contribution is nil. Quantitation of androstenedione may indicate the androgenic activity in women.

 

 

Assay Principle

This is an ELISA for the quantitative analysis of Androstenedione levels in biological fluid.  This test kit operates on the basis of competition between the hormone conjugate and the Androstenedione in the sample for a limited number of binding sites on the antibody coated plate.The sample or standard solution is first added to the microplate.  Next, the diluted hormone conjugate is added and the mixture is shaken and incubated at room temperature for one hour.  During the incubation, competition for binding sites is taking place.  The plate is then washed removing all the unbound material.  The bound hormone conjugate is detected by the addition of substrate which generates an optimal color after 30 minutes. Quantitative test results may be obtained by measuring and comparing the absorbance reading of the wells of the samples against the standards with a microplate reader at 450nm or 650nm.  The extent of color development is inversely proportional to the amount of Androstenedione in the sample or standard.  For example, the absence of Androstenedione in the sample will result in a bright blue color, whereas the presence of Androstenedione will result in decreased or no color development.If using urine as a sample it is recommended to run our

 

 

Creatinine Assay kit (CR01)

in conjunction with your samples since urinary creatinine levels may be used to normalize the rate of excretion of other analytes. 

Cross Reactivity  

Androstenedione        100.00%Estrone                             1.50%Pregnenolone                  0.20%Deoxycorticosterone       0.16%Estrone-3-sulfate             0.16%17b-Estradiol                   0.08%Hydrocortisone                0.08%Prednisolone                   0.08%Estriol                                0.01%

MSDS
EA68.MSDS.pdf
Spec Sheet
EA68.pdf
品牌介绍
Oxford Biomedical Research(OBR)公司由Denis M. Callewaert博士于1984年建立。OBR公司开发,生产和销售检测试剂盒,重组蛋白和应用于生物医学和制药研究的抗体,现已具有400多种产品。我们产品的重点是对引发多种疾病的两大风险因素氧化应激和慢性炎症的生物标志物的识别与检测。我们采用专有技术,开发组织和疾病的特异性生物标志物。