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当前位置: 首页 > 产品中心 > Storage_reagent > 牛津生物医学研究/活性人uPA功能分析试剂盒/试剂盒/PL91
商品详细牛津生物医学研究/活性人uPA功能分析试剂盒/试剂盒/PL91
牛津生物医学研究/活性人uPA功能分析试剂盒/试剂盒/PL91
牛津生物医学研究/活性人uPA功能分析试剂盒/试剂盒/PL91
商品编号: PL91
品牌: oxfordbiomed
市场价: ¥11000.00
美元价: 5500.00
产地: 美国(厂家直采)
公司:
产品分类: 储存液
公司分类: Storage_reagent
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍
Product Overview

Background

Urokinase-type Plasminogen Actvator (uPA) is a glycosylated serine protease that is synthesized in endothelial and kidney epithelial cells. There are two forms of uPA, the low molecular weight (LMW ~31 kDa) and high molecular weight (HMW ~55 kDa). The HMW form undergoes autoproteolysis resulting in the LMW plus and 18.5 kDa amino terminal fragment (ATF). It is this ATF that has been shown to inhibit proliferation and invasion of cancer cells by binding to uPA receptors (1). 

Assay Principle

This is an ELISA for the quantitative analysis of active uPA levels in biological fluid. This test kit operates on the basis of sandwich ELISA where free active uPA enzyme complexes with PAI-1 and is quantitated with the use of an HRP labeled secondary antibody.Provided is biotinylated PAI-1 bound to avidin coated wells. This allows active tPA present in the standard or unknown, to complex with PAI-1. Inactive or complexed uPA is removed in a subsequent wash step. A primary antibody specific for uPA is then added to each well followed by the HRP conjugated secondary antibody. The bound conjugated secondary antibody is detected by the addition of substrate, which generates an optimal color after 10 minutes. Quantitative test results may be obtained by the measure and comparison of the sample and standard absorbance readings when read with a microplate reader at 450 nm.

Spec Sheet
PL91.pdf
品牌介绍
Oxford Biomedical Research(OBR)公司由Denis M. Callewaert博士于1984年建立。OBR公司开发,生产和销售检测试剂盒,重组蛋白和应用于生物医学和制药研究的抗体,现已具有400多种产品。我们产品的重点是对引发多种疾病的两大风险因素氧化应激和慢性炎症的生物标志物的识别与检测。我们采用专有技术,开发组织和疾病的特异性生物标志物。